mouse anti-collagen type iii (Millipore)
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mouse anti-collagen type iii
Mouse Anti Collagen Type Iii, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-collagen+type+iii/rabbit+anti+collagen+iv/pmc11172307-159-31-35
Average 90 stars, based on 1 article reviews
Mouse Anti Collagen Type Iii, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-collagen+type+iii/rabbit+anti+collagen+iv/pmc11172307-159-31-35
Average 90 stars, based on 1 article reviews
mouse anti-collagen type iii - by Bioz Stars,
2026-10
90/100 stars
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1) Product Images from "Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids"
Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids
Journal: International Journal of Molecular Sciences
doi: 10.3390/ijms25115844
Figure Legend Snippet: Effects of ethyl pyruvate on the expression of fibrotic and cytostatic markers in HDFs and KFs. ( A ) Treatment of TGF-β significantly increased the expression of collagen I/III mRNA. Treating ethyl pyruvate significantly decreased mRNA expression as the dose increased. ( B ) Similarly, treating ethyl pyruvate to KFs showed reduced collagen I/III mRNA expression (* p < 0.05).
Techniques Used: Expressing
Figure Legend Snippet: Effect of ethyl pyruvate on expression of fibrosis-related protein. ( A ) Western blot analysis of collagen I, III, TGF-β, and p-SMAD2/3 expression was performed; ( B ) upon densitometric analysis, collagen I, III, TGF-β, and p-SMAD2/3 protein expression levels decreased when KF cells were treated with 20mM of ethyl pyruvate (* p < 0.05).
Techniques Used: Expressing, Western Blot
Figure Legend Snippet: Immunohistochemical analysis of collagen I and III, fibronectin, and elastin in ethyl pyruvate-treated keloid spheroids ( n = 3). ( A ) Representative images of collagen I and III, fibronectin, and elastin IHC staining of keloid spheroids treated with ethyl pyruvate (10, 20, and 40 mM). ( B ) Semi-quantification of protein expression is shown. Collagen I and III, fibronectin, and elastin were significantly decreased in keloid spheroids following ethyl pyruvate application. Asterisks (*) over each column signify a statistically significant difference compared to the control (0 mM, white bar) group (* p < 0.05). ( C ) Reduction in collagen I protein expression can be seen with western blot analysis.
Techniques Used: Immunohistochemical staining, Immunohistochemistry, Expressing, Western Blot
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Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), Article Title: Keratocyte behavior in three-dimensional photopolymerizable poly(ethylene glycol) hydrogels Article Snippet: After labeling cells, constructs were fixed with 3% paraformaldehyde (PFA; EMS, Fort Washington, PA) in serum and phenol red-free DMEM/F-12 for 10 min, followed by 3% PFA in PBS for 20 min. 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Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), Article Title: Keratocyte behavior in three-dimensional photopolymerizable poly(ethylene glycol) hydrogels Article Snippet: After labeling cells, constructs were fixed with 3% paraformaldehyde (PFA; EMS, Fort Washington, PA) in serum and phenol red-free DMEM/F-12 for 10 min, followed by 3% PFA in PBS for 20 min. After rinsing with PBS, samples were quenched with 50 mM Article Title: Composition for preventing or treating keloid or hypertrophic scars Article Snippet: The keloid spheroid sections were treated with mouse anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Western Blot:Article Title: Heat Shock Protein 90 Inhibitor Decreases Collagen Synthesis of Keloid Fibroblasts and Attenuates the Extracellular Matrix on the Keloid Spheroid Model Article Snippet: To evaluate expression of ECM proteins, keloid spheroid sections were incubated at 4°C overnight with the following primary antibodies: mouse, anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Article Title: Quantitative multispectral imaging of Herovici's polychrome for the assessment of collagen content and tissue remodelling. Article Snippet: Bioprosthetic devices, constructed from a variety of materials, are routinely implanted in a variety of anatomical locations.. 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Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), Article Title: Keratocyte behavior in three-dimensional photopolymerizable poly(ethylene glycol) hydrogels Article Snippet: After labeling cells, constructs were fixed with 3% paraformaldehyde (PFA; EMS, Fort Washington, PA) in serum and phenol red-free DMEM/F-12 for 10 min, followed by 3% PFA in PBS for 20 min. After rinsing with PBS, samples were quenched with 50 mM Article Title: Composition for preventing or treating keloid or hypertrophic scars Article Snippet: The keloid spheroid sections were treated with mouse anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Immunohistochemical staining:Article Title: Heat Shock Protein 90 Inhibitor Decreases Collagen Synthesis of Keloid Fibroblasts and Attenuates the Extracellular Matrix on the Keloid Spheroid Model Article Snippet: To evaluate expression of ECM proteins, keloid spheroid sections were incubated at 4°C overnight with the following primary antibodies: mouse, anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Article Title: Quantitative multispectral imaging of Herovici's polychrome for the assessment of collagen content and tissue remodelling. Article Snippet: Bioprosthetic devices, constructed from a variety of materials, are routinely implanted in a variety of anatomical locations.. 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Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), Article Title: Keratocyte behavior in three-dimensional photopolymerizable poly(ethylene glycol) hydrogels Article Snippet: After labeling cells, constructs were fixed with 3% paraformaldehyde (PFA; EMS, Fort Washington, PA) in serum and phenol red-free DMEM/F-12 for 10 min, followed by 3% PFA in PBS for 20 min. After rinsing with PBS, samples were quenched with 50 mM Article Title: Composition for preventing or treating keloid or hypertrophic scars Article Snippet: The keloid spheroid sections were treated with mouse anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Labeling:Article Title: Heat Shock Protein 90 Inhibitor Decreases Collagen Synthesis of Keloid Fibroblasts and Attenuates the Extracellular Matrix on the Keloid Spheroid Model Article Snippet: To evaluate expression of ECM proteins, keloid spheroid sections were incubated at 4°C overnight with the following primary antibodies: mouse, anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Article Title: Quantitative multispectral imaging of Herovici's polychrome for the assessment of collagen content and tissue remodelling. Article Snippet: Bioprosthetic devices, constructed from a variety of materials, are routinely implanted in a variety of anatomical locations.. Essential to their success is the formation of a non-destructive interface with the host tissue and appropriate tissue remodelling.. Traditionally, the main method of assessing the host–material interface has been qualitative histological evaluation, using pattern recognition and comparative assessment to identify changes in the normal tissue architecture that are characteristic of scar tissue. Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), Article Title: Keratocyte behavior in three-dimensional photopolymerizable poly(ethylene glycol) hydrogels Article Snippet: After labeling cells, constructs were fixed with 3% paraformaldehyde (PFA; EMS, Fort Washington, PA) in serum and phenol red-free DMEM/F-12 for 10 min, followed by 3% PFA in PBS for 20 min. After rinsing with PBS, samples were quenched with 50 mM Article Title: Composition for preventing or treating keloid or hypertrophic scars Article Snippet: The keloid spheroid sections were treated with mouse anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Construct:Article Title: Heat Shock Protein 90 Inhibitor Decreases Collagen Synthesis of Keloid Fibroblasts and Attenuates the Extracellular Matrix on the Keloid Spheroid Model Article Snippet: To evaluate expression of ECM proteins, keloid spheroid sections were incubated at 4°C overnight with the following primary antibodies: mouse, anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Article Title: Quantitative multispectral imaging of Herovici's polychrome for the assessment of collagen content and tissue remodelling. Article Snippet: Bioprosthetic devices, constructed from a variety of materials, are routinely implanted in a variety of anatomical locations.. Essential to their success is the formation of a non-destructive interface with the host tissue and appropriate tissue remodelling.. Traditionally, the main method of assessing the host–material interface has been qualitative histological evaluation, using pattern recognition and comparative assessment to identify changes in the normal tissue architecture that are characteristic of scar tissue. Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), Article Title: Keratocyte behavior in three-dimensional photopolymerizable poly(ethylene glycol) hydrogels Article Snippet: After labeling cells, constructs were fixed with 3% paraformaldehyde (PFA; EMS, Fort Washington, PA) in serum and phenol red-free DMEM/F-12 for 10 min, followed by 3% PFA in PBS for 20 min. After rinsing with PBS, samples were quenched with 50 mM Article Title: Composition for preventing or treating keloid or hypertrophic scars Article Snippet: The keloid spheroid sections were treated with mouse anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Sequencing:Article Title: Heat Shock Protein 90 Inhibitor Decreases Collagen Synthesis of Keloid Fibroblasts and Attenuates the Extracellular Matrix on the Keloid Spheroid Model Article Snippet: To evaluate expression of ECM proteins, keloid spheroid sections were incubated at 4°C overnight with the following primary antibodies: mouse, anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Article Title: Quantitative multispectral imaging of Herovici's polychrome for the assessment of collagen content and tissue remodelling. Article Snippet: Bioprosthetic devices, constructed from a variety of materials, are routinely implanted in a variety of anatomical locations.. Essential to their success is the formation of a non-destructive interface with the host tissue and appropriate tissue remodelling.. Traditionally, the main method of assessing the host–material interface has been qualitative histological evaluation, using pattern recognition and comparative assessment to identify changes in the normal tissue architecture that are characteristic of scar tissue. Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), Article Title: Keratocyte behavior in three-dimensional photopolymerizable poly(ethylene glycol) hydrogels Article Snippet: After labeling cells, constructs were fixed with 3% paraformaldehyde (PFA; EMS, Fort Washington, PA) in serum and phenol red-free DMEM/F-12 for 10 min, followed by 3% PFA in PBS for 20 min. After rinsing with PBS, samples were quenched with 50 mM Article Title: Composition for preventing or treating keloid or hypertrophic scars Article Snippet: The keloid spheroid sections were treated with mouse anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Reverse Transcription Polymerase Chain Reaction:Article Title: Heat Shock Protein 90 Inhibitor Decreases Collagen Synthesis of Keloid Fibroblasts and Attenuates the Extracellular Matrix on the Keloid Spheroid Model Article Snippet: To evaluate expression of ECM proteins, keloid spheroid sections were incubated at 4°C overnight with the following primary antibodies: mouse, anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Article Title: Quantitative multispectral imaging of Herovici's polychrome for the assessment of collagen content and tissue remodelling. Article Snippet: Bioprosthetic devices, constructed from a variety of materials, are routinely implanted in a variety of anatomical locations.. Essential to their success is the formation of a non-destructive interface with the host tissue and appropriate tissue remodelling.. Traditionally, the main method of assessing the host–material interface has been qualitative histological evaluation, using pattern recognition and comparative assessment to identify changes in the normal tissue architecture that are characteristic of scar tissue. Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), Article Title: Keratocyte behavior in three-dimensional photopolymerizable poly(ethylene glycol) hydrogels Article Snippet: After labeling cells, constructs were fixed with 3% paraformaldehyde (PFA; EMS, Fort Washington, PA) in serum and phenol red-free DMEM/F-12 for 10 min, followed by 3% PFA in PBS for 20 min. After rinsing with PBS, samples were quenched with 50 mM Article Title: Composition for preventing or treating keloid or hypertrophic scars Article Snippet: The keloid spheroid sections were treated with mouse anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Cell Culture:Article Title: Heat Shock Protein 90 Inhibitor Decreases Collagen Synthesis of Keloid Fibroblasts and Attenuates the Extracellular Matrix on the Keloid Spheroid Model Article Snippet: To evaluate expression of ECM proteins, keloid spheroid sections were incubated at 4°C overnight with the following primary antibodies: mouse, anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Article Title: Quantitative multispectral imaging of Herovici's polychrome for the assessment of collagen content and tissue remodelling. Article Snippet: Bioprosthetic devices, constructed from a variety of materials, are routinely implanted in a variety of anatomical locations.. Essential to their success is the formation of a non-destructive interface with the host tissue and appropriate tissue remodelling.. Traditionally, the main method of assessing the host–material interface has been qualitative histological evaluation, using pattern recognition and comparative assessment to identify changes in the normal tissue architecture that are characteristic of scar tissue. Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), Article Title: Keratocyte behavior in three-dimensional photopolymerizable poly(ethylene glycol) hydrogels Article Snippet: After labeling cells, constructs were fixed with 3% paraformaldehyde (PFA; EMS, Fort Washington, PA) in serum and phenol red-free DMEM/F-12 for 10 min, followed by 3% PFA in PBS for 20 min. After rinsing with PBS, samples were quenched with 50 mM Article Title: Composition for preventing or treating keloid or hypertrophic scars Article Snippet: The keloid spheroid sections were treated with mouse anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Marker:Article Title: Heat Shock Protein 90 Inhibitor Decreases Collagen Synthesis of Keloid Fibroblasts and Attenuates the Extracellular Matrix on the Keloid Spheroid Model Article Snippet: To evaluate expression of ECM proteins, keloid spheroid sections were incubated at 4°C overnight with the following primary antibodies: mouse, anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Article Title: Quantitative multispectral imaging of Herovici's polychrome for the assessment of collagen content and tissue remodelling. Article Snippet: Bioprosthetic devices, constructed from a variety of materials, are routinely implanted in a variety of anatomical locations.. Essential to their success is the formation of a non-destructive interface with the host tissue and appropriate tissue remodelling.. Traditionally, the main method of assessing the host–material interface has been qualitative histological evaluation, using pattern recognition and comparative assessment to identify changes in the normal tissue architecture that are characteristic of scar tissue. Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), Article Title: Keratocyte behavior in three-dimensional photopolymerizable poly(ethylene glycol) hydrogels Article Snippet: After labeling cells, constructs were fixed with 3% paraformaldehyde (PFA; EMS, Fort Washington, PA) in serum and phenol red-free DMEM/F-12 for 10 min, followed by 3% PFA in PBS for 20 min. After rinsing with PBS, samples were quenched with 50 mM Article Title: Composition for preventing or treating keloid or hypertrophic scars Article Snippet: The keloid spheroid sections were treated with mouse anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Imaging:Article Title: Heat Shock Protein 90 Inhibitor Decreases Collagen Synthesis of Keloid Fibroblasts and Attenuates the Extracellular Matrix on the Keloid Spheroid Model Article Snippet: To evaluate expression of ECM proteins, keloid spheroid sections were incubated at 4°C overnight with the following primary antibodies: mouse, anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Article Title: Quantitative multispectral imaging of Herovici's polychrome for the assessment of collagen content and tissue remodelling. Article Snippet: Bioprosthetic devices, constructed from a variety of materials, are routinely implanted in a variety of anatomical locations.. Essential to their success is the formation of a non-destructive interface with the host tissue and appropriate tissue remodelling.. Traditionally, the main method of assessing the host–material interface has been qualitative histological evaluation, using pattern recognition and comparative assessment to identify changes in the normal tissue architecture that are characteristic of scar tissue. Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), Article Title: Keratocyte behavior in three-dimensional photopolymerizable poly(ethylene glycol) hydrogels Article Snippet: After labeling cells, constructs were fixed with 3% paraformaldehyde (PFA; EMS, Fort Washington, PA) in serum and phenol red-free DMEM/F-12 for 10 min, followed by 3% PFA in PBS for 20 min. After rinsing with PBS, samples were quenched with 50 mM Article Title: Composition for preventing or treating keloid or hypertrophic scars Article Snippet: The keloid spheroid sections were treated with mouse anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Produced:Article Title: Heat Shock Protein 90 Inhibitor Decreases Collagen Synthesis of Keloid Fibroblasts and Attenuates the Extracellular Matrix on the Keloid Spheroid Model Article Snippet: To evaluate expression of ECM proteins, keloid spheroid sections were incubated at 4°C overnight with the following primary antibodies: mouse, anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), Article Title: Quantitative multispectral imaging of Herovici's polychrome for the assessment of collagen content and tissue remodelling. Article Snippet: Bioprosthetic devices, constructed from a variety of materials, are routinely implanted in a variety of anatomical locations.. Essential to their success is the formation of a non-destructive interface with the host tissue and appropriate tissue remodelling.. Traditionally, the main method of assessing the host–material interface has been qualitative histological evaluation, using pattern recognition and comparative assessment to identify changes in the normal tissue architecture that are characteristic of scar tissue. Article Title: Ethyl Pyruvate Decreases Collagen Synthesis and Upregulates MMP Activity in Keloid Fibroblasts and Keloid Spheroids Article Snippet: For IHC staining, the keloid spheroid sections were incubated overnight at 4 °C with primary antibodies including mouse anti-HMGB1 (Abcam), mouse anti-AKT (Abcam), mouse anti-ERK1/2 (Abcam), mouse anti-collagen type I (Abcam), Article Title: Keratocyte behavior in three-dimensional photopolymerizable poly(ethylene glycol) hydrogels Article Snippet: After labeling cells, constructs were fixed with 3% paraformaldehyde (PFA; EMS, Fort Washington, PA) in serum and phenol red-free DMEM/F-12 for 10 min, followed by 3% PFA in PBS for 20 min. After rinsing with PBS, samples were quenched with 50 mM Article Title: Composition for preventing or treating keloid or hypertrophic scars Article Snippet: The keloid spheroid sections were treated with mouse anti-collagen type I (ab6308; Abcam, Ltd., Cambridge, UK), |